Apolipoprotein C-III-1 activates lysosomal sphingomyelinase in vitro.

Apolipoprotein C-III-1 activates lysosomal sphingomyelinase in vitro.

Apolipoprotein (apo)C-III-1 from human very low density lipoprotein stimulates 14-fold the activity of lysosomal sphingomyelinase from human fibroblasts. At the sphingomyelin concentrations tested, maximal stimulation was obtained with 5 microM apoC-III-1 or apoC mixture. Apolipoproteins A-I, A-II, B, and C-I conferred little or no stimulation. Sphingomyelinase was stimulated 20-fold by lysopho...

Lysosomal Sphingomyelinase 1053

ISection for Medical Enzymology and Metabolism, Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 lid Amsterdam, The Netherlands; 2Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagkstrasse i, D-5300 Bonn, Federal Republic of Germany; and 3Developmental and Metabolic Neurology Branch, National Institute of Neurological and Communicative Di...

Lipids: Apolipoprotein A-1 Apolipoprotein A-II Apolipoprotein B Apolipoprotein C-II Apolipoprotein C-III Apolipoprotein E Cholesterol Direct HDL Cholesterol Direct LDL Cholesterol Lipoprotein (a) sLDL Triglycerides

Apolipoprotein C-I induces apoptosis in human aortic smooth muscle cells via recruiting neutral sphingomyelinase.

OBJECTIVE Apolipoprotein C-I (apoC-I) influences lipoprotein metabolism, but little is known about its cellular effects in aortic smooth muscle cells (ASMC). METHODS AND RESULTS In cultured human ASMC, apoC-I and immunoaffinity purified apoC-I-enriched high-density lipoproteins (HDL) markedly induced apoptosis (5- to 25-fold), compared with control cells, apoC-I-poor HDL, and apolipoprotein C...

Apolipoprotein C-III bound to apolipoprotein B-containing lipoproteins in obese girls.

taining Lipoproteins in Obese Girls, Piers R. Blackett, Robin Germany, Boureima Sambo, and Petar Alaupovic, ( Department of Pediatrics, 2 University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104; 3 Medical Research Foundation, Oklahoma City, OK 73109; * address correspondence to this author at: Children’s Hospital of Oklahoma, Room 2426, 940 NE 13th St., Oklahoma City, OK 73104; f...